Figure 5.

TH⁺ mDA axon projections to the intermediate region of the lateral septum are specifically missing in adult Neurod6 mutant mice. A, B, TH immunohistochemistry shows specific loss of axon projections (arrowheads) to the LSi but not to LSd in Neurod6 mutant adult mice. C, D, TH⁺ axon projections from mDA neurons have not yet reached the dorsal region of the lateral septum (LS) at E18.5. E–J, TH⁺ mDA neuronal projections to other target sites of the VTA, including nucleus accumbens (NAc), olfactory tubercle (OT), amygdala (Amg), and prefrontal cortex (PFC), appear normal in Neurod6 mutant compared with control adult mice. TH⁺ axons of SNc mDA neuronal projections to the striatum (Str) were also unaffected. MS, Medial septum; LSv, lateral septum, ventral; cc, corpus callosum; ac, anterior commissure. G–J, Insets, Higher magnification of boxed region. Scale bars: A, 200 μm.