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. 2017 Mar 1;37(9):2305–2316. doi: 10.1523/JNEUROSCI.2414-16.2016

Figure 8.

Figure 8.

Neurod1 is also required for the survival of Neurod6+ mDA neurons. A–F, YFP+/TH+ mDA neurons are lost at E18.5 in Neurod1;Neurod6 double mutants carrying different copy numbers of Neurod1 and Neurod6 mutant alleles. Immunohistochemistry of YFP and TH shows that reduced numbers of YFP+/TH+ mDA neurons are observed in coronal sections of the central midbrain in double-mutant embryos carrying 2 (C), 3 (D, E), or 4 Neurod1;Neurod6 mutant alleles (F). In contrast, Neurod6 single-homozygous mutants present no change in YFP+/TH+ expression in mDA neurons at E18.5 (B). G, Bar graph represents percentage changes of the number of YFP+/TH+ mDA neurons in the central mDA region of Neurod1;Neurod6 double and Neurod6 single-mutant embryos normalized to Neurod6Cre/+ control embryos. Error bars indicate SEM; N = 3 per genotype. *p < 0.05 (one-way ANOVA, with Tukey's post test). **p < 0.01 (one-way ANOVA, with Tukey's post test). ***p < 0.0001 (one-way ANOVA, with Tukey's post test). Scale bars: A, 100 μm.