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Isolation of macrophage-specific mRNA from the spinal cord injury site. A–C, In uninjured spinal cord, hemagglutinin (green) is expressed in a minor population of Iba1+ microglia and dendritic cells (red). D–F, At 7 d after SCI, HA is expressed in the majority of myeloid cells at the injury core. Immunohistochemistry was performed on lysMRpl22(HA) mice. All images are sagittal sections of the midthoracic spinal cord. Scale bar, 500 μm. n = 3 biological replicates per group.
