Figure 2.

Myeloid cells labeled in lysM-Cre mice are representative of the general macrophage population after SCI. A–T, We performed SCI on lysM^tdTom→WT chimera mice and assessed the injury site using flow cytometry to determine whether macrophages labeled in lysM^tdTom mice (CD11b⁺/tdTom⁺; B, red) were representative of the general macrophage population in the injury site (CD11b⁺/CD45^hi; A, circled region). Except for a slight difference in Ly6C⁺ cells at 14 d after SCI, macrophages labeled in lysM^tdTom (red bars) mice did not differ from the general macrophage population (gray bars) in the percentage of cells that expressed Ly6G, CD11c, CD86, CD206, or CD43 at both 7 and 14 d after SCI. *p < 0.05 using Student's unpaired t test. n = 5 biological replicates per group. Error bars indicate SEM.