Fig 10. Ex vivo porcine liver (top panel) and cardiac muscle tissue (bottom panel) sonicated with 600 W at 5 Hz PRF.

Top Panel: a. Gross pathology of the lesion appeared thermally denatured, with a diameter of 8 mm (blue dotted circle). This area was not structurally intact and disintegrated during pathological stain preparation process. b. H&E slide showing diffuse thermal effects, with little cellular structure. c. Magnification (40X) of the Fig 10b with parts of the lesion showing absence of cellular structure, representing homogenization of tissue. Bottom Panel. d. Cardiac muscle with a liquefied void in the center of the lesion with sharp boundary with negligible thermal effects (red arrowhead). e. H&E slide of the lesion shows that cellular structure around the void was intact, with no evidence of thermal damage. The lesion also has sharp boundaries (red arrowhead). f. Magnification (40X) of the lesion boundary, with most of the boundary tissue intact.