Fig 5. c-KIT overexpression in c-KIT+ transfected cells.
Flow cytometric analysis of c-KIT expression in control, empty vector transfected cells and c-KIT overexpressing cells. Data are represented in dot plots of GFP (FL1) vs. SSC and CD117-PE (FL2) vs. SSC. Non-transfected cells (A1-2), empty vector transfected cells (B1-2) and c-KIT overexpressing cells (C1-2). Blue regions in A1, B1 and C1 plots represent the percentage of GFP fluorescent cells (transfection efficiency). Green gates in A2, B2 and C2 plots show red fluorescent cells labeled with PE antibody (c-KIT expression).