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. 2017 Mar 6;13(3):e1006266. doi: 10.1371/journal.ppat.1006266

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© 2017 Xu et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — (A) Cell cycle analysis. NS1-S1 cells were transduced with shRNA-expressing lentivirus as indicated. After 48 h, cells were treated with Dox at 5 μg/ml (Dox+) or without (Dox-). After 72 h, the cells were collected and co-stained with an anti-BrdU antibody and DAPI. Cell cycle analysis of mCherry-expressing cells is shown. (B) Statistical analyses. The percentage of cells at G1-, S-, and G2-phase after shRNA transduction is depicted in color. The numbers show the percentages of the cells at G2-phase. The percentage of shScram and other shRNAs transduced NS1-expressing cells at G2-phase was statistically analyzed. **P<0.01, and N.S. represents no significance.