Fig 1. Tn5 and Sleeping Beauty Transpositional Transgenesis in bovine and ovine.

A) Schematic depiction of the plasmids used for in vitro transposition with reporter transposons in bovine zygotes. B) Schematic depiction of the plasmids used for in vivo transposition with rhFIX transposons in ovine zygotes. The Tn5 protein was complexed to the transposon in vitro, in Mg⁺² free medium. The resulting transposome was used for injection into presumptive zygotes. The mixture of SB plasmids was deposited directly into the cytoplasm of the zygotes.