Table 3. Summary of Tn5 and Sleeping Beauty transpositional transgenesis in vivo in ovine.

Treatment	Rep.	N° injected zygotes	N° transferred zygotes	N° pregnant/ recipients	N° fetuses (tz) [r]	N° born sheep (tz) [r]	N° tg born sheep (tz) [r] {b}
ME(rhFIX):Tn5	1	15	12	2/3	2 (16) [66.6]	1 (8.3)[33.3]	0 (0) [0] {0}
	2	9	9	0/2	0 (0)	0 (0)	0 (0) [0] {0}
	Total	24	21a	2/5	2 (9.5) [40]	1 (4.7) [20]	0 (0) [0] {0}
pT2/rhFIX-pCMV-SB100X	1	62	21	3/7	4 (19) [57]	4 (19) [57]	1*(4.7)[14.2]{25}
	2	45	12	1/4	1 (8.3)[25]	1 (8.3)[25]	1 (8.3)[25]{100}
	3	34	18	2/6	2 (11.1)[33.3]	2 (11.1)[33.3]	0 (0) [0] {0}
	Total	141	51b	6/17	7 (13.7)[41.1]	7 (13.7)[41.1]	2 (3.9)[11.7]{28.6}

^a different superscripts in the same column indicate significant differences among treatments (Fisher test; p<0.05).

^b different superscripts in the same column indicate significant differences among treatments (Fisher test; p<0.05).

ME(rhFIX):Tn5: rhFIX Tn5 transposome. pT2/rhFIX-pCMV-SB100X: rhFIX transposon/ Sleeping Beauty transposase plasmids. Rep.: repetition. Tg: transgenic. The rate of fetuses, born sheep and transgenic born sheep were calculated over number of transferred zygotes (tz), and over number of recipient ewes [r]. The rate of transgenic born sheep was also calculated over the total number of born animals{b}.

*the transgene was only detected in liver and placenta.