Fig 4. Adaptation to CD4^low allows efficient infection of macrophages.

(A) Two types of macrophages, M-MDM and G-MDM expressing differential CD4 levels (S4A Fig) were infected with 0.1ng p24 of ultra-centrifuged Env-pseudotyped luciferase reporter virus stocks infection measured on day seven post-infection by quantifying luciferase reporter activity (relative light units (RLU)). Data are means from two individual donors, input of ultracentrifugation purified virus was standardized by p24 content, error bars = SD. (B) Infection of M-MDM and G-MDM by CD4^low adapted viruses relative NAB01-PA (data derived from A. (C) Comparison of M-MDM and G-MDM infectivity. Shown is the relative infectivity of G-MDM compared to M-MDM infection (data derived from A).