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. 2017 Mar 16;12(3):e0174038. doi: 10.1371/journal.pone.0174038

Fig 1. GTL001 binding to the human CD11b/CD18 receptor.

Fig 1

(A) Human PBMC were incubated with or without biotinylated CyaA-HPV16 E7, followed by fluorescently labeled antibodies. Bound biotinylated CyaA-HPV16 E7 was detected with PE-streptavidin and analyzed by flow cytometry. Results are the means of triplicate determinations ± standard deviation. (B) CHO-CR3 CD11b+ cells were incubated with biotinylated CyaA-HPV16 E7 and 0 to 512 nM CyaA, the solution formulation of GTL001, CyaA-HPV16 E7, or CyaA-HPV18 E7. After washing, bound biotinylated CyaA was detected with PE-labeled streptavidin and flow cytometry. Results are from a single experiment in which all substances were compared but are representative of two to six experiments per tested substance.