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. 2017 Mar 16;12(3):e0174038. doi: 10.1371/journal.pone.0174038

Fig 3. Induction of HPV E7-specific CD8+ T-cell responses as measured by cytometric bead array.

Fig 3

C57BL/6 mice (5/group) were vaccinated intradermally with 10 μg of the solution formulation of GTL001 or placebo (PBS with 1.83 M urea) on day 0. Immediately and 24 h after vaccination, 25 mg of 5% imiquimod cream/ear was applied at each vaccination site as indicated. Splenocytes were collected on day 7, pooled, and stimulated ex vivo without or with 30 μg/mL synthetic HPV16 E7 antigen (HPV16 E743-98). After 3 days, production of IFN-γ, IL-6, TNF-α, and IL-4 was assessed by cytometric bead array. Values are from single determinations per splenocyte pool. IL-4 does not appear in several cases because it was undetectable.