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. 2017 Feb 2;292(10):4210–4221. doi: 10.1074/jbc.M116.767897

FIGURE 7.

FIGURE 7.

Dominant negative effect of the external loops on the channel activity of gain-of-function TRPP2_F604P channel and TRPP3-PKD1L3 complex. A, representative current-voltage curves of the currents of the indicated proteins expressed in Xenopus oocytes, indicating that the coexpression of TRPP2 S1-S2 loop inhibits the current of TRPP2_F604P. B, scatter plot showing that the TRPP2 loop exerts a dominant negative effect on the current of TRPP2_F604P. **, p < 0.01. Currents at +60 mV are shown. Mean and S.D. are shown with purple bars. C, representative current-voltage curves of acid-induced off-response currents in Xenopus oocytes expressing indicated proteins, revealing that the coexpression of either PKD1L3 S6-S7 loop or TRPP3 S1-S2 loop inhibits the current of the TRPP3-PKD1L3 complex. D, scatter plot showing that both PKD1L3 and TRPP3 loops exert a dominant negative effect on the acid-induced current of the TRPP3-PKD1L3 complex. Currents at +60 mV are shown. ***, p < 0.001. Error bars, S.D.