FIGURE 1.

ADAR3 expression is consistent with an inhibitor of GRIA2 editing in astrocyte- and grade IV astrocytoma-derived cell lines. A, chromatograms of the Q/R site of GRIA2 in NHA, U87, and U118 cells. Black indicates transcripts with guanosine (edited), and green indicates transcripts with adenosine (unedited). B, editing levels at the Q/R site of GRIA2 were measured in the indicated cell lines (n = 2). Error bars represent S.E. C, equivalent amounts of cell lysates were determined by Bradford assay and subjected to SDS-PAGE and immunoblotting for ADAR2. Tubulin is the loading control. D, lysates from NHA cells transduced with retrovirus containing a neomycin resistance vector with no protein (Emp.) or human ADAR2 expressed from the CMV promoter (OE) were subjected to SDS-PAGE and immunoblotted for ADAR2. Tubulin is the loading control. E, chromatograms of the Q/R site of GRIA2 in the cell lines show in D. Black indicates transcripts with guanosine (edited), and green indicates transcripts with adenosine (unedited). Bold A indicates the genomically encoded adenosine at the Q/R site of GRIA2. F and G, equivalent amounts of cell lysates were determined by Bradford assay and subjected to SDS-PAGE and immunoblotting for ADAR1 (F) or ADAR3 (G). Tubulin is the loading control.