FIGURE 2.
RECQL4 physically interacts with RPS3. A, nuclear lysates of 293 cell lines stably expressing RECQL4-V5-his or control plasmid are used for V5 pulldown followed by anti-RPS3, anti-V5 and anti-Lamin B immunoblotting. B, Coomassie Blue-stained SDS-PAGE depicting final purified protein fraction of GST-FL-RPS3 after BiorexTM70 and glutathione-Sepharose purification steps. C, GST pulldown assay. Anti-RECQL4 and anti-GST blots show input and pulldown fractions of GST pulldown assay carried out by incubating RECQL4-his with GST-RPS3 or GST only proteins. D, confocal images of representative U2OS cells immunostained by anti-RECQL4, anti-RPS3 antibodies, and DAPI. The plot shows nuclear RPS3 (%) and nuclear colocalization of RPS3-RECQL4 (%) quantified from three-dimensional reconstructions of Z section confocal images, using Imaris software. The data are shown as the means ± S.D. of triplicate experiments.