FIGURE 5.

Chronic exposure to MPO promotes a dose- and time-dependent cytotoxicity. A, MPO promotes a dose-dependent cytotoxicity in ARPE-19 cells. Cells were incubated with increasing doses of MPO for 8 days (3 cultures per concentration), then analyzed for cell viability by high content imaging. B, MPO promotes a time-dependent cytotoxicity in ARPE-19 cells. Cells were incubated with MPO for up to 20 days (3 cultures per time point) then analyzed for cell viability by high content imaging. In A and B, cells were fixed and stained with Hoechst. High content images were acquired at 40× magnification using the blue channel to visualize nuclei as an indication of cell numbers per image (four sites per well). Results in A and B are representative of three repeat experiments.