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Binding of AdoCbl to ATR. A, changes in the UV-visible absorption spectrum upon binding of free AdoCbl (20 μm, black trace) to 3–30 μm ATR (final spectrum, red trace) in Buffer A. The absorption maximum shifts from 525 to 456 nm with isosbestic points at 388 and 481 nm. The data presented here are representative of at least three independent experiments. B, modulation of AdoCbl binding by nucleotides. The data represent the mean ± S.D. of three independent experiments.
