Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Kinetics parameters for E166H hydrolyzing penicillin G and cephaloridine
| β-Lactamase | Antibiotics | Km | kcat | kcat/Km |
|---|---|---|---|---|
| μm | s−1 | m−1s−1 | ||
| WT | Penicillin G | 60.9 ± 5.3 | 393.8 ± 18.5 | (6.5 ± 0.9) × 106 |
| Cephaloridine | 20.7 ± 4.1 | 8.2 ± 0.8 | (4.0 ± 1.0) × 105 | |
| E166H | Penicillin G | 149.4 ± 38.2 | 0.46 ± 0.07 | (3.1 ± 1.0) × 103 |
| Cephaloridine | 4.7 ± 0.8 | 0.022 ± 0.001 | (4.7 ± 1.1) × 103 |
