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. 2017 Jan 30;292(10):3988–4002. doi: 10.1074/jbc.M116.763243

FIGURE 6.

FIGURE 6.

Ca2+/CaM-inhibition of wild-type CPK28 and the F344E mutant. Representative determinations of the IC50 for Ca2+/CaM inhibition of CPK28 (A) or the F344E mutant (B). Assays were performed at 100 μm CaCl2, 100 μm ATP, and 10 μm ACSM+1 peptide. CaM inhibition is shown on a relative scale where 100% inhibition corresponds to an ∼60% loss of activity compared with untreated protein.