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. 2017 Jan 30;292(10):3988–4002. doi: 10.1074/jbc.M116.763243

TABLE 3.

Phosphopeptides identified from in situ autophosphorylated recombinant CPK28

Data shown are from representative peptide identifications; all product ion spectra were inspected manually to ensure correct phosphosite identification.

Sitea Mr (experimental) Mr (calculated) E-value Peptide Fragmentation Ref.
Ser-6 988.4201 988.4202 0.0041 M.GVCFpSAIR.V HCD
Ser-24 1207.5859 1207.5849 4.2e-07 K.pSKAAPTPIDTK.A HCD 16
Thr-33 1379.6712 1379.6697 0.00032 K.AAPTPIDpTKASTK.R HCD 16
Ser-36 1379.6718 1379.6697 0.00095 K.AAPTPIDTKApSTK.R HCD 16
Thr-33/Thr-37 1615.7376 1615.7372 0.00043 K.AAPTPIDpTKASpTK.R ETD
Ser-43 898.3620 898.3620 0.0014 R.TGpSIPCGK.R HCD 16
Ser-198 1037.4808 1037.4794 1.0e-05 K.pSAQLDSPLK.A HCD
Ser-228 1986.9549 1986.9492 5.1e-10 R.FHDIVGpSAYYVAPEVLK.R HCD 16, 17, 27
Ser-318 1502.7392 1502.7395 6.0e-05 R.LTAAQALpSHAWR.E HCD 16, 17, 27
Thr-329 2075.9707 2075.9888 0.0016 R.EGGNApTDIPVDISVLNNLR.Q HCD
Ser-360 1682.7788 1682.7764 9.5e-09 R.ALApSTLDEAEISDLR.D HCD
Tyr-463b 1271.5453 1271.5435 0.0036 K.DGpYITPEELR.M HCD 16
Ser-478 1607.7088 1607.7080 3.9e-06 R.GpSIDPLLDEADIDR.D HCD -
Ser-495 1280.5914 1280.5914 0.00058 R.DGKIpSLHEFR.R HCD 17, 27
Ser-510 928.4015 928.4015 0.0002 R.TASISpSQR.A HCD
Ser-510 1701.7954 1701.7948 3.0e-10 R.TASISpSQRAPSPAGHR.N ETD
Ser-507/Ser-510 1008.3676 1008.3678 0.039 R.TApSISpSQR.A HCD
Ser-507/Ser-510 1781.7618 1781.7611 0.00013 R.TApSISpSQRAPSPAGHR.N ETD

a Sites listed in boldface are new sites identified only in this study.

b Tyr(P)-463 was only identified in samples from protein expressed with the addition of CaCl2 to the expression culture at the time of induction.