TABLE 4.

Oligonucleotide primers used in this study

Name	Restriction site	Sequencea	Purpose
CPK28_F_NdeI	NdeI	atcgcatatgGGTGTCTGTTTCTCCGCC	pET28a cloning
CPK28_R_NotI	NotI	atcggcggccgcCTATCGAAGATTCCTGTGACC	pET28a cloning
CPK11_F_NdeI	NdeI	atcgcatatgGAGACGAAGCCAAACCCTAGACG	pET28a cloning
CPK11_R_NotI	NotI	atcggcggccgcTCAGTCATCAGATTTTTCACC	pET28a cloning
CPK13_F_NdeI	NdeI	atcgcatatgGGAAACTGTTGCAGATCTCCCGC	pET28a cloning
CPK13_R_NotI	NotI	atcggcggccgcCTATTCGTTGCCTAGGTTC	pET28a cloning
CPK16_F_NheI	NheI	atcggcatgcATGGGTCTCTGTTTCTCCTCC	pET28a cloning
CPK16_R_NotI	NotI	atcggcggccgcTTAGACCTTGCGAGAAATAAGATAACC	pET28a cloning
S360A		CAATTTGCTTTAAGGGCGCTTGCTgcCACACTTGACGAGGCAGAGATCTC	CPK28 mutagenesis
S360Arc		GAGATCTCTGCCTCGTCAAGTGTGgcAGCAAGCGCCCTTAAAGCAAATTG	CPK28 mutagenesis
F344E		CATTTCAGTTCTGAACAACTTAAGACAAgaaGTGAGATACAGCCGTCTAAAGC	CPK28 mutagenesis
F344Erc		GCTTTAGACGGCTGTATCTCACttcTTGTCTTAAGTTGTTCAGAACTGAAATG	CPK28 mutagenesis
F353E		GAGATACAGCCGTCTAAAGCAAgaaGCTTTAAGGGCGCTTGCTAGC	CPK28 mutagenesis
F353Erc		GCTAGCAAGCGCCCTTAAAGCttcTTGCTTTAGACGGCTGTATCTC	CPK28 mutagenesis

^a Underlined residues deviate from the wild-type CPK28 sequence for directed mutagenesis.