Figure 2. Dam1 and Ndc80 complexes interact through three distinct sites.
(A) Average microtubule residence time of the wild-type Ndc80-GFP complex alone, in the presence of mock treated, phosphorylated Dam1 complex, or phosphorylated Dam1 6A mutant complex. Dam1 6A: all six Dam1 complex phosphorylation sites mutated to Ala. (B) Average microtubule residence time of wild-type Ndc80-GFP complex in the presence of selectively phosphorylated Dam1 complex. Diagram on the left indicates phosphorylated proteins. (C) Average microtubule residence time of Ndc80-GFP complex with lethal mutation in region ANdc80p, BNdc80p, or CNdc80p in the presence of selectively phosphorylated Dam1 complex. Diagrams on the left indicate phosphorylated protein (P) and Ndc80p region with a lethal mutation (∧). On the right are representative kymographs for each experiment. Bars represent average residence time ± error of the mean (estimated by bootstrapping analysis; see Materials and methods or additional details). Ndc80-GFP complex microtubule residence time raw data are included in Figure 2—source data 1. Refer to Supplementary file 1C for statistical analysis.
DOI: http://dx.doi.org/10.7554/eLife.21069.010
Figure 2—figure supplement 1. Microtubule residence time survival probability plots of experiments in Figure 2.
Figure 2—figure supplement 2. Mutating Dam1 complex Aurora B kinase phosphorylation sites to Ala does not affect the interaction between Dam1 and Ndc80 complexes.
