Figure 4. The Ndc80^10hep complex does not support growth.

(A) Red/white plasmid shuffle assay to test the viability of the Ndc80^10hep complex. Solid red colonies indicate the inability of the Ndc80^10hep complex and empty vector to support growth. Sectoring white colony indicates the ability of the wild-type Ndc80 complex to support growth. (B) α-FLAG immunoblot of crude lysate of cells expressing either wild-type Ndc80p, Ndc80p^10hep, or empty vector. α-actin immunoblot of the same lysates shows equal loading of the lysates. (C) Wild-type or Ndc80^10hep complex average residence time (top) on microtubules in the absence and presence of Dam1 complex as derived from the survival probability curves (bottom). Bars represent average residence time ± error of the mean (estimated by bootstrapping analysis; see Materials and methods or additional details). Ndc80-GFP complex microtubule residence times raw data are included in Figure 4—source data 1. Refer to Supplementary file 1F for statistical analysis of data.

DOI: http://dx.doi.org/10.7554/eLife.21069.020