Appendix 1—table 4. Costs of lipids.

The average cost per molecule is calculated for a variety of species using estimates of lipid compositions from the literature and the formulas described in the text. The fraction of fatty acids of given length and saturation level is not shown. Cardiolipin costs are assumed to be 637 (evolutionary) and 236 (reduced) ATP. The cost for molecules in the ‘other’ category is assumed to be the average of glycerophospholipids (GPL) in the species and cardiolipin.

		GPL cost		Composition			Mean cost
Species	Membrane	Tot.	Red.	GPL	Cardiolipin	Other	Tot.	Red.		References
Escherichia coli	Whole cell	367	115	0.926	0.060	0.015	385	124		Haest et al. (1969); Rietveld et al. (1993); Raetz et al. (1979)
Bacillus subtilis	Whole cell	308	102	0.818	0.183	0.000	368	127		Bishop et al. (1967); López et al. (1998)
Caulobacter crescentus	Whole cell	340	111	0.776	0.105	0.119	389	132		Contreras et al. (1978); Chow and Schmidt (1974)
Staphylococcus aureus	Whole cell	323	105	0.931	0.070	0.000	345	114		Haest et al. (1972); Mishra and Bayer (2013)
Zymomonas mobilis	Whole cell	370	118	0.990	0.010	0.000	373	119		Carey and Ingram (1983)
							372	123	mean
							8	3	SE
Candida albicans	Whole cell	338	123	0.934	0.066	0.000	358	131		Goyal and Khuller (1992); Singh et al. (2010)
Chlamydomonas reinhardtii	Whole cell	390	140	0.935	0.065	0.000	406	146		Janero and Barrnett (1981); Giroud et al. (1988); Tatsuzawa et al. (1996)
Debaryomyces hansenii	Whole cell	408	141	0.913	0.087	0.000	428	150		Kaneko et al. (1976)
Dictyostelium discoideum	Whole cell	400	141	0.965	0.014	0.000	395	139		Davidoff and Korn (1963); Ellingson (1974); Weeks and Herring (1980); Paquet et al. (2013)
Paramecium tetraurelia	Whole cell	415	146	0.996	0.004	0.000	415	146
Pichia pastoris	Whole cell	412	144	0.975	0.025	0.000	418	147		Klug et al. (2014)
Saccharomyces cerevisiae	Whole cell	372	133	0.953	0.047	0.000	385	138		Longley et al. (1968); Kaneko et al. (1976); Sharma (2006); Klis et al. (2014)
Schizosaccharomyces pombe	Whole cell	411	142	0.945	0.055	0.000	424	147		Koukou et al. (1990)
							403	143	mean
							8	2	SE
Debaryomyces hansenii	Plasma membrane	398	137	0.913	0.087	0.000	418	146		Kaneko et al. (1976); Turk et al. (2007)
Dictyostelium discoideum	Plasma membrane	414	145	0.980	0.020	0.000	418	147		Weeks and Herring (1980)
Dunaliella salina	Plasma membrane	378	137	1.000	0.000	0.000	378	137		Peeler et al. (1989); Azachi et al. (2002)
Mus musculus , thymocytes	Plasma membrane	409	142	0.921	0.000	0.079	418	145		Van Blitterswijk et al. (1982)
Saccharomyces cerevisiae	Plasma membrane	358	129	0.949	0.035	0.026	375	135		Longley et al. (1968); Zinser et al. (1991); Swan and Watson (1997); Tuller et al. (1999); Blagović et al. (2005)
Schizosaccharomyces pombe	Plasma membrane	411	142	0.856	0.052	0.092	433	151		Koukou et al. (1990)
Vigna radiata , seedling	Plasma membrane	402	141	1.000	0.000	0.000	402	141		Yoshida and Uemura (1986)
							406	143	mean
							8	2	SE
Candida albicans	Mitochondrion	344	125	0.710	0.164	0.126	411	150		Goyal and Khuller (1992)
Danio rerio , whole fish	Mitochondrion	472	162	0.854	0.104	0.042	492	172		Almaida-Pagán et al. (2014)
Pichia pastoris	Mitochondrion	421	145	0.944	0.054	0.002	433	150		Wriessnegger et al. (2009); Klug et al. (2014)
Rattus norwegicus , liver	Mitochondrion	445	154	0.838	0.148	0.024	480	169		Tahin et al. (1981); Colbeau et al. (1971)
Saccharomyces cerevisiae	Mitochondrion	312	116	0.897	0.097	0.006	345	128		Tuller et al. (1999); Zinser et al. (1991); Blagović et al. (2005)
Serripes groenlandicus , gill	Mitochondrion	428	147	0.972	0.028	0.000	434	150		Gillis and Ballantyne (1999)
Sus scrofa , heart	Mitochondrion	409	143	0.797	0.186	0.017	453	161		Comte et al. (1976)
Tetrahymena pyriformis	Mitochondrion	402	144	0.812	0.131	0.057	439	159		Gleason (1976); Nozawa (2011)
							436	155	mean
							16	5	SE