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. 2016 Dec 29;8(6):10145–10160. doi: 10.18632/oncotarget.14358

Figure 8. Effect of AMOT on YAP target gene expression and the involvement of BMP4 in AMOT-mediated PCa cell proliferation.

Figure 8

A. PC-3mm2 cells overexpressing AMOTp80, AMOTp130, or both, and C4-2B4 cells with knock-down of AMOT by pGIPZ control vector, shAMOT RNA #1 or #2 in pGIPZ vectors. YAP target genes were detected by real-time PCR using specific primers. B. Left panel, PC3-mm2 cells overexpressing AMOTp80, p130, or both were incubated with an increasing concentration of LDN193189, a BMP Type I receptor antagonist, for two days and the number of cells was counted. Right panel, PC3-mm2 cells overexpressing AMOTp80, p130, or both were treated with 500 nM LDN193189. C. Left panel, C4-2B4 cells with AMOT knockdown were treated with an increasing concentration of LDN193189 and the number of cells was counted. Right panel, C4-2B4 cells with AMOT knockdown were treated with 200 nM LDN193189. D. Working model of AMOTp80-mediated PCa cell proliferation. AMOTp80 likely interacts with Merlin and this leads to decreases in the levels of MST1, pLATS and pYAP. The ensuing decrease in YAP phosphorylation leads to an increase in the nuclear translocation of YAP. YAP activation stimulates the transcription of BMP4, resulting in an increase in PCa cell proliferation.