Table 3. The primer sequences of COL1A1, COL3A1 and GAPDH for qRT-PCR.
Gene | Primer sequence (5′-3′) |
---|---|
COL1A1 | F: TCAAGATGGTGGCCGTTACT |
R: CATCTTGAGGTCACGGCATG | |
COL3A1 | F: TGGGATGCAACTACCTTGGT |
R: AGGTGTAGAAGGCTGTGGAC | |
GAPDH | F: GTGACAGCCGCATCTTCTTG |
R: TGACTGTGCCGTTGAACTTG |
Note: qRT-PCR, quantitative real-time polymerase chain reaction; GAPDH, glyceraldehyde-3- phosphate dehydrogenase; F, forward; R, reverse.