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. 2016 Dec 30;8(6):10213–10224. doi: 10.18632/oncotarget.14378

Figure 2. EZH2 inhibition induces the expression of miR-125a and miR-320c that are predicted to target IRF-4, XBP-1 and BLIMP-1.

Figure 2

A. miRNAs predicted by miRNA.org resource to target IRF-4, XBP-1 and BLIMP-1 and to be upregulated by EZH2 inhibition in the MM INA-6 cell line. Heatmap analysis shows changes in expression of miR-125a and miR-320c in four independent biological experiments. INA-6 cell line was treated with 1μM of UNC1999 for 5 days. QRT-PCR validation of primary miRNA B. and mature miRNA C. in two MM cell lines, INA-6 treated with 1 μM of UNC1999 and LP-1 treated with 4 μM of UNC1999 for 5 days. Upregulation of miR-494 that is known to target c-MYC was detected by qRT-PCR but not in mature miRNA array. RNU6B was used as housekeeping miRNA. DMSO was used as control treatment. Error bars represent standard deviation of three independent biological experiments. P-values were calculated using student t-test GraphPad prism, P-value: *≤ 0.05; **≤0.01; ***≤0.001.