Figure 4. Infection of primary cells from a respiratory epithelium with multiple EBV strains.

A. We infected four different batches of primary cells from the respiratory epithelium with supernatants containing the same numbers of infectious particles from the indicated EBV strains and stained them for EBER expression 3 days post-infection. The cells were also submitted to an immunostain with an antibody specific for keratin and the nuclei were counterstained with DAPI. The percentage of infected cells is given in the adjacent graph of bars and we show the results of 4 infections performed with samples from independent individuals. The arrows point to EBER-positive cells. B. Same experiment as in (A) except that we used transfer infection. C. The primary samples used in (A) and (B) were exposed to viral supernatants. After extensive washings, we quantified the number of copies bound to the target cells. The results are given relative to signals obtained with M81. The p-values shown in (A) and (B) give the results of global mixed linear model analyses with random effect. In (C) we applied log-transformation of fold-changes for the analysis of normalized data without Bonferroni correction. Error bars represent the mean with s.d.