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. 2017 Jan 2;8(6):10400–10415. doi: 10.18632/oncotarget.14422

Figure 4. HSPB8 silencing and its effect on expression of ERα and ERβin MCF-7 cell line.

Figure 4

MCF-7 cells were transfected with siRNA-HSPB8 or NT-siRNA and the analysis was performed 1, 2, 3, 4 and 5 days after transfection. The HSPB8 mRNA and protein levels were measured by real-time RT-PCR A. and Western blot analysis B. Statistical analysis was performed by one-way ANOVA followed by Bonferroni multiple comparison tests *p<0.05 vs Control-1day (untransfected cells). Representative pictures of immunofluorescence staining of HSPB8 (red, anti-rabbit) and α-tubulin (green, anti-mouse) in MCF-7 cells transfected for 1 and 2 days. DAPI (blue) was used to stain DNA C. NT = Non-targeting. ERα D. and ERβ E. mRNA levels were measured by real-time RT-PCR analysis 1, 2, and 3 days after transfection. Values represent the mean from three independent experiments, no statistical differences were observed. The expression of different small heat shock protein was evaluated by Western blot analysis in MCF-7 cell line F. MCF-7 cells were transfected with siRNA-HSPB1 or NT-siRNA and HSPB1 protein levels were measured by Western blot analysis 3, 4 and 5 days after transfection G. Statistical analysis was performed by one-way ANOVA followed by Bonferroni multiple comparison tests *p<0.05 vs Control-1day (untransfected cells).