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. 2017 Jan 2;8(6):10400–10415. doi: 10.18632/oncotarget.14422

Figure 5. FACScan analysis of MCF-7 cells.

Figure 5

The adherent MCF-7 cells were treated with transfection medium (Control-1 and -2) A-C. transfected with siRNA-HSPB8 B., with siRNA-HSPB1 D., with empty vector as control E. and with plasmid pCI-NEO-HSPB8 that contains the sequence of human HSPB8 cloned in pCI F. After 3 days, the cells were measured on a FACScan flow cytometer with an argon laser at 488 nm for excitation and analyzed using Cell Quest software. Each experiment was performed in triplicate. The efficiency of HSPB8 overexpression in MCF-7 cells was evaluated by Western blot analysis, 1, 2 and 3 days after transfection G. Statistical analysis was performed by one-way ANOVA followed by Bonferroni multiple comparison tests *p<0.05 vs Control-1day (untransfected cells); EV empty vector.