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. 2017 Jan 2;8(6):10400–10415. doi: 10.18632/oncotarget.14422

Figure 7. MCF-7 cells proliferation and migration.

Figure 7

MCF-7 cells were transfected with siRNA-HSPB8 A. or siRNA-HSPB1 B. and cellular proliferation was evaluated by MTT assay 1, 4 and 5 days after transfection. MCF-7 cells were transfected with siRNA-HSPB8 and treated for 5 days after transfection with 10nM 17β-estradiol C. 1μM 3β-Adiol D. and 1μM genistein E. Control: cells untransfected and treated with DMSO. Cellular proliferation was evaluated 1, 2, 3, 4 and 5 days after transfection. Statistical analysis was performed by two-way ANOVA followed by Bonferroni multiple comparison tests. Values represent the mean from three independent experiments. *p<0.01vs Control; #p<0.01 17β-estradiol vs siRNA-HSPB8+17β-estradiol; §p<0.01 3β-Adiol vs siRNA-HSPB8+3β-Adiol; $p<0.01 genistein vs siRNA-HSPB8+genistein. For the migration assay, MCF-7 untransfected cells were treated for 2 days with DMSO (first column), while the cells transfected with siRNA-HSPB8 were treated for 2 days after transfection with DMSO (second column), or with 17β-estradiol (E, 10nM), 3β-Adiol (3β, 1μM) and genistein (Gen, 1μM). Cells were then detached, and transferred to Boyden’s Chamber F. Statistical analysis was performed by one-way ANOVA followed by Bonferroni multiple comparison tests. Values represent the mean from three independent experiments. *p<0.05 vs untransfected cells (first column); #p<0.05 vs 17β-estradiol.