Figure 2. Analysis of the formation of acini in 3D cultures over time.

Cells were treated with E2 or E2 plus baicalein (8 μM) for 5 weeks, and were then allowed to grow in Matrigel for 12 days. Images were captured at 0, 4, 8, and 12 days after seeding under a microscope at 400× magnification. The photographs are representative of three independent experiments.