Table 1. FoxO1 functions on different myoblast differentiation stage.

Myoblasts mold	Functions on myoblast differentiation		Conclusion	Year	Reference
	Inhibits myoblast early differentiation	Required for myoblast fusion
Mouse primary myoblasts		Yes	FoxO1 is required for mouse primary myoblast fusion	2003	[21]
C2C12 myoblasts	Yes		An active form of FoxO1 mutant inhibits C2C12 cell differentiation whereas an inactive mutant FoxO1 can partially restore inhibition of C2C12 cell differentiation regulated by wortmannin	2003	[33]
C2C12 myoblasts		Yes	Inactivation of Rho/ROCK signaling is crucial for myoblast fusion and nuclear translocation of FoxO1	2004	[49]
C2C12 myoblasts		Yes	Negative-feedback loop between FoxO1a and cGKI fine-tunes the progress of muscle cell fusion process	2005	[76]
C2C12 myoblasts	Yes		Interaction between FoxO1 and Notch1 inhibits myoblast differentiation through promoting corepressor clearance and recruiting the coactivator of Csl, leading activation of Hes family, which is considered to be a myoblast differentiation repressor	2007	[25]
C2C12-RasV12/C2C12-RasV12C40 myoblasts	Yes		Nuclear exclusion of FoxO1 is required for C2C12-RasV12C40 myoblast differentiation	2008	[43]
C2C12 myoblasts	Yes		FoxO1 negatively regulates myoblast differentiation through degradation of mTOR pathway components	2008	[52]
L6 myoblasts		Yes	Inhibition of FoxO1 transcriptional activity or nuclear exclusion of FoxO1 suppresses L6-mIRS1 cell differentiation	2011	[22]
C2C12 myoblasts	Yes		PAX3/FOXO1A and PAX7/FOXO1A suppress myogenesis through inhibiting transcriptional activity of MyoD-target genes	2013	[70]
Rhabdomyosarco-ma cells	Yes		PAX3-FOXO1 fusion protein inhibits rhabdomyosarcoma cell differentiation through upregulating JARID2	2014	[74]
C2C12 myoblasts	Yes		Insulin triggers FoxO1 nuclear exclusion and protein degradation to reverse inhibited myogenesis by FoxO1	2014	[35]