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. 2017 Jan 3;8(6):9035–9052. doi: 10.18632/oncotarget.14764

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Copyright: © 2017 Zhu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Splenic DCs were isolated with CD11c⁺ MicroBeads from mice model. Apoptosis rate of splenic DCs was determined by flow cytometric analysis. Sal (1mg/kg) was administered at 6 h after thermal injury. The apoptosis rates were assessed using the sum of area UR (Annexin-V⁺/7-AAD^- indicates late apoptotic cells) and area LR (Annexin-V⁺/7-AAD^- indicates early apoptotic cells). Results were shown in mean ± SD (n = 6). * Statistically significant difference when compared with sham group at the same time point (p < 0.05). & Statistically significant difference when compared with thermal-injured group (p < 0.05).