Figure 1. SORBS1 is present at a lower level in human breast cancer.

(A) Western blots was performed to detect SORBS1 levels in normal human mammary gland epithelial cell line MCF 10A and nine other human breast cancer cell lines. (B) Box plots comparing levels of SORBS1 mRNA in normal human breast tissues and breast carcinomas (left panel) or ductal breast carcinoma tissues (right panel) in published data sets from Oncomine. ***P < 0.001, Student's t-test. (C) mRNA levels of SORBS1 in normal breast tissues vs invasive ductal / lobular breast carcinomas tissues in the case of Curtis Breast, or mRNA levels of SORBS1 in ductal breast carcinomas vs invasive ductal breast carcinoma in Nikolsky Breast in published data sets from Oncomine. *P < 0.05, Student's t-test. (D and E) Kaplan-Meier survival analysis for assessment of overall survival (OS) (D) and distant metastasis-free survival (DMFS) (E) in breast cancer patients with (right panel) or without (left panel) systemic treatment which classified by relative (high or low) tumor SORBS1 level. Survival curves were generated by using the Kaplan-Meier Plotter online tool based on data stratified based on the best cut-off. Curves were compared by hazard ratios (HR) and p values (log rank p).