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. 2016 Dec 21;8(6):9303–9322. doi: 10.18632/oncotarget.14066

Figure 6. A single pulse of BAF A1 transiently affects stemness markers in senescent HCT116 cells.

Figure 6

(A–D) Evaluation of percentage of CD24 positive cells in cultures treated with the AFTER CHEMO or the AFTER CHEMO + BAF A1 protocol at various time points. Cells stained with H33342, as a ploidy discriminator, were probed with an anti-CD24-FITC antibody. Percentages of all cells (A), and CD24+ cells with 2C&4C (B), 8C (C) and 16C (D) DNA content were determined by flow cytometry. Cells labeled with isotypic IgGs were used as a negative control. (E–H) Evaluation of percentage of CD133+ cells at various time points. Cells stained with H33342 were probed with an anti-CD133-APC antibody. Percentages of all cells (E), and CD133+ cells with: 2C&4C (F), 8C (G) and 16C (H) DNA content were determined by flow cytometry. (I–L) Evaluation of percentages of CD44+ cells at various time points. Cells stained with H33342, were incubated with an anti-CD44-AlexaFluor700 antibody. Percentages of all cells (I), and CD44+ cells with: 2C&4C (J), 8C (K) and 16C (L) DNA content were determined by flow cytometry. Each bar represents mean ± SEM, N ≥ 3.*p < 0.05, **p < 0.01, ***p < 0.001 – AFTER CHEMO vs. AFTER CHEMO + BAF A1.