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. 2016 Dec 26;8(6):9739–9751. doi: 10.18632/oncotarget.14185

Figure 4. Blockade of the CCL2–CCR2 or CCL17/22–CCR4 axis inhibits the migration of prostate cancer cells.

Figure 4

Prostate cancer cells are incubated with a CCR2 antagonist (CCR2 ant; 20 μg/ml) or CCR4 antagonist (CCR4 ant; 10 μg/ml) for 30 min and then incubated with CCL2 (10–30 ng/ml), CCL17 (10–30 ng/ml), CCL22 (10–30 ng/ml), or CM of U937 or U937-M cells for 24 h for PC-3 and DU145 and 48 h for LNCaP. The mean optical density (OD) value is read using a microreader at 595 nm. Data are presented as mean ± SD. All experiments are performed in triplicate, and mean values are shown. *p < 0.05, **p < 0.01, ***p < 0.001.