Skip to main content
. 2016 Dec 26;8(6):9739–9751. doi: 10.18632/oncotarget.14185

Figure 6. The CCL2–CCR2 and CCL22–CCR4 axes increase phosphorylation of Akt.

Figure 6

A. Phosphorylation of Akt (Ser473) proteins in prostate cancer cells is assayed using western blot at 30 min after CCL2, CCL17, or CCL22 stimulation (left panels) and after CCR4 antagonist treatment with CCL2, CCL17, or CCL22 stimulation (right panels). B. U937 cell-induced phosphorylation of Akt (Ser473) proteins in prostate cancer cells with or without CCR2 and CCR4 antagonists is also assayed using western blot. Adjustments of brightness, contrast, and size are applied to the whole images of western blot-based analyses without elimination of any information present in the original, including backgrounds. C. PC-3 cells are incubated with the Akt inhibitor AZD5363 (10 μg/ml) with CCL17 (30 ng/ml) and CCL22 (30 ng/ml) for 24 h. The mean optical density (OD) value is read using a microreader at 595 nm. Data are presented as mean ± SD. All experiments are performed in triplicate, and mean values are shown. **p < 0.01. All experiments are performed in triplicate, and representative data are shown.