. 2016 Dec 30;8(3):3781–3797. doi: 10.18632/oncotarget.14389

Table 1. Sequences of primers used in the real-time PCR.

Name		Sequence(5′–3′)
GAPDH (Rat)	Forward	ACTAACCCTGCGCTCCTG
GAPDH (Rat)	Reverse	CCCAATACGACCAAATCAGA
Aggrecan (Rat)	Forward	CTTCCCAACTATCCAGCCAT
Aggrecan (Rat)	Reverse	TCACACCGATAGATCCCAGA
Col1α1 (Rat)	Forward	CGTGGAAACCTGATGTATGC
Col1α1 (Rat)	Reverse	GGTTGGGACAGTCCAAGTCT
Col2α1 (Rat)	Forward	CGAGGCAGACAGTACCTTG
Col2α1 (Rat)	Reverse	TGCTCTCGATCTGGTTGTTC
MMP9 (Rat)	Forward	GCTCATCCTACCCATTGCAT
MMP9 (Rat)	Reverse	GCTTCCCTGTCATCTTCAGC
MMP3 (Rat)	Forward	TCCTTGCAATGTGGATGTTT
MMP3 (Rat)	Reverse	CGTCCTTGAAGAAATGCAGA

GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; Col1α1: Type I collagen (Col I); Col2α1: Type II collagen (Col II); MMP1: Matrix metallopeptidase-1; MMP3: Matrixmetallopeptidase-3; MMP9: Matrix metallopeptidase-9.