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. 2016 Jun 4;8(3):3826–3839. doi: 10.18632/oncotarget.9830

Figure 6. TGFBR-Deficiency Increases Tumor Associated Inflammation in Conditional APC Δ468 Driven Colon Carcinogenesis.

Figure 6

a.,b. Tissue sections from four-month old cAPC and cATG mice were stained with H&E and tumor infiltrating leukocyte infiltration scored by two investigators. Sections were also dual-stained with CD3/E-Cadherin to identify T cells, CD3/E-Cadherin to label regulatory T cells (Tregs), or F4/80/E-Cadherin to identify macrophages and scored similarly. c. The presence of live CD4+FoxP3+CD25+ Tregs in the mesenteric lymph nodes of cAPC and cATG mice was detected via flow cytometry. d.,e. Spleens were harvested from nongenic cAPC and cATG animals, and analyzed for expression of the myeloid marker CD11b and GR1, a marker of most inflammatory granulocytes, showing a pronounced increase in CD11b+GR1+ cells in cATG mice. f. Gating to GR1+CD11b+ populations in cAPC and cATG mice revealed increased F4/80+ splenic macrophages in cATG. (*, P < 0.05. N = 4 per group).