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. 2016 Nov 10;8(3):4051–4061. doi: 10.18632/oncotarget.13266

Figure 3. Effects of K8 on cell cycle and apoptosis in glioblastoma cell lines 8401 and G2T.

Figure 3

(A) The cells were treated with K8 80 uM for 0, 24, 48 hrs and analyzed for cell distribution by flow cytometry. (B–C) The subG1 and G2/M cell population were quantified. (D–E) 8401, G2T were treated with K8 20, 40, 80 uM for 24 hrs and then analyzed for AnnexinV-PI double stain to detect for cell apoptosis. (F) After K8 treatment of 8401 and G2T cell lines apoptosis-related protein was expressed. Experiments were carried out at least three times. Level of statistical significance: *P < 0.05, **p < 0.01.