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. 2016 Dec 1;8(3):4196–4207. doi: 10.18632/oncotarget.13755

Figure 5. Identification of Bmi18 as a direct target of miR-218 in OCSCs.

Figure 5

(A) miR-218 expression in andrographolide-treated OCSCs; (B) Schematic representation of the constructed Bmi1 3′UTR reporter plasmids; (C) The wild-type or mutant form of Bmi1 reporter was co-transfected with miR-218 or empty vector (Mock) into OCSCs. The luciferase activity was assessed and presented as relative units to Mock-treated cells; (D) mRNA expression of miR-218 and (E) protein expression of Bmi1 in OCSCs transfected with pLV-miR-scrambled (pLV-miR-Scr.) or pLV-miR-218; (F) Survival fraction of pLV-miR-Scr or pLV-miR-218-treated OCSCs after various doses of radiation. Experiments were performed in triplicate. Values are expressed as mean ± SD. *p < .05 compared to control.