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. 2016 Nov 7;8(3):4484–4500. doi: 10.18632/oncotarget.13152

Figure 1. ARHGEF2 is a transcriptional target of oncogenic KRAS.

Figure 1

A. Western blot analysis of ARHGEF2 in the indicated PDAC cell lines following acute knockdown of KRAS with the increasing concentrations of siRNA. Lysates were probed with indicted antibodies 72 hours post transfection. For this and subsequent experiments, tubulin served as a protein loading control. B. QPCR analysis of ARHGEF2 mRNA in the indicated cell lines treated with control siRNA or siRNA targeting KRAS (10nM final concentration). Error bars in this and subsequent qPCR experiments represent standard deviations from three independent experiments. C. QPCR analysis of ARHGEF2 mRNA expression in normal pancreas (Normal) and patient-derived xenografts (Tumor). The p-value represents significance between the normal and tumor group. D. Western blot and QPCR analysis of ARHGEF2 in Panc-1 and MiaPaCa-2 cell lines treated with the indicated concentration of U0126 (U0). Lysates were probed with indicted antibodies 48 hours post treatment. E. Western blot and QPCR analysis of ARHGEF2 in Panc-1 and MiaPaCa-2 cell lines treated with the indicated concentration of LY294002 (LY). Lysates were probed with indicted antibodies 48 hours post treatment.