Skip to main content
. 2016 Nov 7;8(3):4484–4500. doi: 10.18632/oncotarget.13152

Figure 3. Identification and validation of transcription factors regulating the minimal ARHGEF2 promoter.

Figure 3

A. Summary of putative transcription factor (TF) binding elements predicted in the AP-15 sequence. Locations of indicated TF binding sites are mapped to their respective location in AP-15 (colored boxes) and numbered relative to the ARHGEF2 TSS (+1). The sequence of each element found in the endogenous promoter (ARHGEF2) and the bases mutated in the AP-15 mutant promoters (Mutant) are shown. B. Normalized luciferase activity generated from AP-15 and AP-14 promoter constructs (blue bars) or AP-15 promoters containing mutations in the indicated TF binding elements (pink bars) transfected in Panc-1 cells. Numbers in parenthesis refer to location of specific TF elements. C. Normalized luciferase activity generated from AP-15 promoter constructs or AP-15 promoters containing a mutant of the indicated TF binding element expressed in the indicated cell lines. D. Normalized luciferase activity generated from the AP-15 promoter construct in Panc-1 cells transfected with control siRNA (siCon) or siRNA targeting the indicated TFs.