Figure 3. miR-182 enhances an epithelial-like state in breast cancer cells.
A. Western blot analysis of E-cadherin and Vimentin in MCF-10A cells transfected with either miR-182 mimics or inhibitor. B-C. The expression of E-cadherin and Vimentin in MDA-MB-231 cells transfected with miR-182 mimics (B) or in MCF-7 cells transfected with miR-182 inhibitor (C) was detected by Western blot. D. Western blot of E-cadherin, Vimentin and SNAI1 protein levels in MCF-10A cells transfected with a control or SNAI1 together with a NC mimics or miR-182 mimics. β-actin is shown as a loading control. The histogram on the right side represents the folder change of each gene expression detected by Western blot, compared with paralleled β-actin.