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. 2016 Nov 24;8(3):4718–4729. doi: 10.18632/oncotarget.13578

Figure 2. Prussian blue-stained and nucleus fast red-counterstained T24 cells incubated with SPIO-R11 or SPIO.

Figure 2

A. T24 cells were incubated with SPIO-R11 at an iron concentration of 50μg/mL of growth medium for indicated time intervals (0 to 12 h). Nanoparticle uptake increased for longer incubation times, especially at the time range of 0-4 h. B. T24 cells were incubated with various iron concentrations of the SPIO-R11 (0 μg/mL, 25 μg/mL, 50 μg/mL, 100 μg/mL and 200 μg/mL) and SPIO (200 μg/mL) for 4 h. A dose-dependent and higher uptake of SPIO-R11 (blue granules) compared with SPIO clearly indicated. The bar in the bottom right corner represents 50 μm.