Figure 14. Effect of osthole on the nuclear translocation of NF-κB.

A. in RAW 264.7 cell; B. in HK-2 cells. The free NF-κB/p65 in nuclear extracts was assessed using the ELISA-based NF-κB kit 24 h after stimulation. Untreated group is set as 100%. Results are expressed as fold increase over untreated group. Data are represented as mean±SD of three independent experiments. ###p< 0.001 compared to untreated group, *p< 0.05, **p< 0.01 and ***p< 0.001 compared to LPS alone.