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. 2016 Dec 3;8(3):5048–5056. doi: 10.18632/oncotarget.13790

Figure 1. FOXD3 knockdown increased human colon cancer cell proliferation in human colon cancer cells.

Figure 1

A. Western blotanalysis of FOXD3 protein blanket expressionin in NCM460, HCT116 and Caco-2 cells. B. HCT116 and Caco-2 cells were transfected with blanket siRNA against FOXD3 or the negative control (non-targeting) siRNA for 72 hours. FOXD3 mRNA was measured by blanket Real time PCR. n=3, *p < 0.05. C. Western blot blanket analysis of FOXD3 protein blanket expression in HCT116 and Caco2 cells treated with scrambled siRNA (control) or FOXD3 siRNA. D and E. After 72 hours post transfection with siRNA against FOXD3 or the negative control (non-targeting) siRNA, HCT116 and Caco-2 cell viability was monitored by MTT assay at the indicated times. F and G. Soft-agar colony formation and statistical analysis for HCT116 cells (C) and Caco-2 cells (D) transfected with scrambled siRNA (negative control, NC) and FOXD3-siRNA, respectively. The experiments were repeated independently for three times. Results represent mean ± SD (n = 6).*p<0.05, **p<0.01, ***p<0.001, siControl versus siFOXD3.