Figure 5. FOXD3 inhibited EGFR/Ras/Raf/MEK/ERK signal pathway in xenograft tumor and human samples.
A. Effects of FOXD3 knockdown on growth of HCT116 subcutaneous xenografts in vivo. Nude mice were injected with 106 cells and tumors were removed at two weeks post injection; each experimental group contained 5 tumors. B. Total protein was isolated from mouse tumors and analyzed by immunoblotting with the antibodies against EGFR, K-Ras and p-ERK. C. The semi-quantitative analysis of FOXD3 immunohisochemical staining in human normal colon and cancer tissues. D. Immunohistochemical analysis of FOXD3, EGFR, K-Ras, p-BRAF, p-MEK, and p-ERK expression in human normal (upper) and colon cancer (lower) tissues. Typical fields of view are presented (Scale bar, 50μm; original magnification,×20).