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. 2016 Dec 3;8(3):5057–5068. doi: 10.18632/oncotarget.13789

Figure 4. CREB can rescue the phenotypes caused by miR-433-3p.

Figure 4

A. Western blot analysis of CREB in U251 and U87 cells co-transfected with either miR-433-3p mimics or scramble and pCMV6/CREB or pCMV6 empty vector. B. and C. The transfected cells were submitted to MTT and colony formation assays to test the proliferation of U251 and U87 cells after co-transfection with mimics and vectors. D. and E. Apoptosis and cell cycle analysis were employed in U251 and U87 cells co-transfected with miR-433-3p mimics and pCMV6/CREB or their control oligomers and vectors. F. and G. Cell motility was examined by transwell invasion and migration assay after co-transfection with mimics and vectors. All experiments were repeated three times. All data are shown as mean ± SD. *P < 0.05 versus Scramble + pCMV6. #P < 0.05 versus miR-433-3p + pCMV6.